Use of plant growth promoting rhizobacteria pgpr is a promising strategy to improve the crop production under optimal or suboptimal conditions. In the present study, five diazotrophic salt tolerant bacteria were isolated from the roots of a halophyte, arthrocnemum indicum. Halotolerant extremophile bacteria from the great salt lake for recycling pollutants in microbial fuel cells. The halotolerant nature of the strain was studied by growing them at different concentrations of nacl. Besides hydrogen and reduced sulfur compounds, bacteria within the chlorobiaceae can utilize only a limited number of organic substrates overmann 2006. Halotolerant extremophile bacteria from the great salt lake. Following that, colonies were picked and restreaked on the same agar medium to obtain pure cultures. Most halophilic and halotolerant fungi described to date from solar salterns have been identified either as known foodborne species with previously unrecognized natural niches, or as species that were not known.
Isolation of bacteria the bacteria were isolated by serial dilution method in which 1 g soil was added in 9ml of dw and diluted from 101 to 106. Similarly, polyethylenes has been reported to degrade in the presence of. References this alphaproteobacteriarelated article is a stub. Two rodshaped, slightly halophilic and extremely halotolerant bacterial strains x1125t and x1174, which were gramstainpositive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of tibetan antelopes. Isolation of halophilic and halotolerant bacteria from a. Of 124 isolates, 102 were grampositive while 22 were gram negative. The peptidoglycan layer is thick effect of dye do not retain the crystal violet dye, and react only with a counterstain, generally stain pink. Peanut arachis hypogaea l is an important cash crop of the leguminous family, grown in most of the arid, and semiarid regions. How do halotolerant microbes differ from halophiles. To study the biodiversity of halophilic bacteria in a protected wetland located in loukkos northwest, morocco, a total of 124 strains were recovered from sediment samples from a marsh and salterns.
All grew well at 5% nacl, but only 25% isolates showed growth at 20% nacl concentration. Research on the halophilic and halotolerant bacteria often seems to be less glamorous than the study of the archaea, with their unique adaptations, including a highly saline. Paradigm for salt stress adaptation and osmoprotection priyanka das1,2, bijay kumar behera1, dharmendra kumar meena1, syed afrin azmi2, soumendranath chatterjee2, kanti meena3 and anil prakash sharma1 1central inland fisheries research institute, barrackpore, kolkata. Salt stress tolerant genes in halophilic and halotolerant. Metal tolerance in halotolerant bacteria publish your. These bacteria are gram positive spore formers and have been identified as belonging to the genus bacillus. Basic laboratory procedures in clinical bacteriology pdf 188p this book is published by world health organization. Although moderately halophilic bacteria are often found on salted fish, meat, and other food products and regardless of their possible involvement in microbial spoilage at high salt concentrations, systematic studies on the occurrence of such bacteria on fish and meat products have rarely been performed.
C for 24 hrs and further pure isolates were obtained from it. In this book who brings together and updates the various guidelines produced by who over the years on sampling of specimens for laboratory investigation, identification of bacteria, and testing of antimicrobial resistance. Interest is increasing in the application of pgprs plant growth promoting rhizobacteria to ameliorate stresses such as salinity stress in crop production. Introduction this section defines the procedures and techniques used to correctly identify the target bacterial. Five strains sl3, sl32, sl35, j8w and pu62 growing well in 20% nacl concentrations were further characterized for multiple plant growth promoting traits such as. Apr 19, 2016 identifying of novel halophilic and halotolerant bacteria that promote plant growth can be used as alternatives for salt sensitive plants. A mesophilic, aerobic, facultatively chemolithoautotrophic bacterium, designated strain epr70t, was isolated from hydrothermal fluids from diffuseflow vents on the east pacific rise at 50. Outlines for the definition of halotolerancehalophily in. Diversity and eps production potential of halotolerant. Viswalingam abstract marine microbes represent a potential source for commercially important bioactive compounds and their bioremediation capabilities are also remarkable. Beside the former answers halophilic halotolerant bacteria have several mechanisms to keep its osmotic pressure despite salt outside its cell walls. Bacteria and bluegreen algae are prokaryotes, while fungi. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate naci concentration up to 15%. Isolation and characterisation of halotolerant bacteria and.
Moreover, it may increase the crop productivity of coastal saline lands in bangladesh by transferring the salt tolerant genes into the plants. Author links open overlay panel matteo grattieri a milomir suvira b kamrul hasan a shelley d. Isolation of halotolerant and halophilic bacteria and. Free bacteriology books download ebooks online textbooks. Hampir semua bakteria yang dipencilkan tergolong di dalam genus bacillus. The effect of the application of halotolerant microorganisms.
Isolation and identification of halotolerant soil bacteria. Eighty four halotolerant bacterial strains were isolated from the saline habitats and screened for growth at different nacl concentrations. Halotolerant bacteria in the effiorescences of a deteriorated. Eukaryote cell difference between prokaryotic and eukaryotic cells. The main groups of halotolerant and halophilic fungi. Pdf media and conditions for the growth of halophilic.
Five strains sl3, sl32, sl35, j8w and pu62 growing well in 20% nacl concentrations were further characterized for multiple plant growth promoting traits such as indole. Salinity stress is one of the major abiotic stresses limiting crop production in arid and semiarid regions. A microorganism is considered extremely halotolerant if its growth range extends above 2. An archive of our own, a project of the organization for transformative works. Chapter 5 bacteriology jason woodland usfws pinetop fish health center pinetop, arizona.
Halotolerant do not require but can adapt to high salt concentrations 2. The consortium was developed using four bacteria, selected on the basis of. Extensive research has been conducted on several halophilic and halotolerant bacterial strains to investigate their plant growth promoting activities. Isolation and characterization of halotolerant aerobic. Halophilic and halotolerant microorganisms from soils. Microbiology module morphology and classification of bacteria microbiology 2 notes zmost bacteria possess peptidoglycan, a unique polymer that makes its synthesis a good target for antibiotics zprotein synthesis takes place in the cytosol with structurally different ribosomes fig. Halotolerant bacteria are able to grow in environments with a wide range of salinities, from 1 to 33% nacl, as well as in the absence of nacl larsen, 1986. Based on the difference in cellular organization and biochemistry, the kingdom protista has been divided into two groups namely prokaryotes and eukaryotes. Metal tolerance in halotolerant bacteria assistant professor vijay kothari priyanka solanki scientific study biology micro and molecular biology publish your bachelors or masters thesis, dissertation, term paper or essay. Isolation and characterisation of halotolerant bacteria. Their optimal temperature, nacl concentration and ph for growth were 28 c, 3 % wv nacl and ph 7. Pdf salt stress is one of the devastating factors that hampers growth and productivity of soybean.
Halotolerance is the adaptation of living organisms to conditions of high salinity. Pdf media and conditions for the growth of halophilic and. The effect of the application of halotolerant microorganisms on the efficiency of a pilotscale constructed wetland for saline wastewater treatment milana karajic1, ales lapanje2, jaka razinger2, alexis zrimec2 and danijel vrhovsek1 1limnos company for applied ecology, podlimbarskega 31, ljubljana and. A gramstainpositive, halotolerant, neutrophilic, rodshaped bacterium, strain mf38t, was isolated from a salinealkaline soil in china and subjected to a polyphasic taxonomic characterization. Besides the two marine isolates, which are both purple nonsulfur. Compared to the extensive literature on the physiology, biochemistry, and ecology of the aerobic red halophilic archaea family halobacteriaceae, the aerobic halophilic bacteria have been relatively little studied. Ventosa a, sanchezporro c, martin s and mellado e 2005 halophilic archaea and bacteria as a source of extracellular hydrolytic enzymes.
Some bacteria can cause diseases for human, animals and plants. Halophiles are organisms that live in highly saline environments, and require the salinity to survive, while. Most halophilic and halotolerant fungi described to date from solar salterns have been identified either as known foodborne species with previously unrecognized natural niches, or as species that were not known to science, and consequently were newly described. The study focused on the isolation and identification of the halotolerant bacteria from three soil samples, collected from. Isolation and characterization of halotolerant aerobic bacteria from oil reservoir beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Mining halophytes for plant growthpromoting halotolerant.
Previous question next question get more help from chegg. Alleviation of salt stress by halotolerant and halophilic. Sneathiella is a halotolerant, aerobic and chemoheterotrophic genus of bacteria from the family of sneathiellaceae. Halotolerant alkalophilic and indolacetic acid producing bacteria associated with arthrospira platensis cyanophyceae scieloabstract growth of a. Halotolerant species tend to live in areas such as hypersaline lakes, coastal dunes, saline deserts, salt marshes, and inland salt seas and springs. A large number of research projects have been focused on finding and classifying novel halophiles. The isolate grew in the presence of 015 % wv nacl and at ph 6. Gram negative bacteria will cause the koh solution to. The bacterial strains have been investigated for ammonia, indole3acetic acid and 1aminocyclopropane1carboxylatedeaminase production, phosphate solubilisation and nitrogen fixation activities.
The possibility to utilize halotolerant extremophile bacteria from the great salt lake as bioelectrocatalysts both for the anodic and the cathodic reactions in single chamber microbial fuel cells has been demonstrated. Media and conditions for the growth of halophilic and halotolerant bacteria and archaea. A prerequisite for molecular studies is the identification of genes involved in the accumulation of compatible solutes. Halotolerant rhizobacteria promote growth and enhance.
In the current study, 18 halotolerant and halophilic bacteria have been investigated for their plant growth promoting abilities in vitro and in a hydroponic culture. Oct 30, 2017 halotolerant bacteria have multiple uses viz. Outside, and closely covering this, lies the rigid, supporting cell wall. Characteristic gramnegative bacteria grampositive bacteria wall structure they have a thin lipopolysaccharide exterior cell wall. Read download file report abuse halophilic and halotolerant microorganismsan citeseerx examples of salttolerant bacteria are found among the aerobic spore formers, many of which grow at salt concentrations up to 15% or even 20%.
What are the characteristics of a halotolerant bacteria. Halotolerant microbial consortia able to degrade highly. Nwfhs laboratory procedures manual second edition, june 2004 chapter 5 page 2 i. The samples were withdrawn at 4h interval and the optical density at 600nm was used to monitor the bacterial growth.
High incidence of halotolerant bacteria in pacific hydrothermalvent and pelagic environments. From 1 g of each of the five samples collected, over 1. The production of peanut has shown to be repeatedly hampered by various abiotic stresses, such as high salt. Greensulfur bacteria belonging to the family chlorobiaceae are strict anaerobes and obligate phototrophs. Because no one media is known to support the growth of all the halotolerant and halophilic bacteria, the generalpurpose, complete media was used for both enrichments and direct plate streaking. Salt stress tolerant genes in halophilic and halotolerant bacteria. A paper by ndwigah in 2016 isolated a halophilic fungus, determining its optimal growth conditions and its metabolites. Cover different classification schemes for grouping bacteria, especially the use of the gram stain 2. Isolation, characterization of halotolerant bacteria and its biotechnological potentials dr. Mitigation of salt stress in wheat seedlings by halotolerant. It can be found in soil, air, water, and living bodies. Eukaryotic and prokaryotic microorganisms, including fungi, bacteria, and archaea, are able to adapt to a range of changes in external osmolarity ruppel et al. Methanogenic enrichments capable of degrading polyethylene glycol and ethylene.
May 05, 2017 beside the former answers halophilic halotolerant bacteria have several mechanisms to keep its osmotic pressure despite salt outside its cell walls. Grampositive bacteria have thick, dense, relatively nonporous walls, while gramnegative bacteria have thin walls surrounded by lipidrich membranes. Bacterial cell morphology, staining reactions, classification of bacteria the protoplast is bounded peripherally has a very thin, elastic and semipermeable cytoplasmic membrane a conventional phospholipid bilayer. Gundecimerman a, oren a, plemenitas a eds adaptation of life at high salt concentrations in archaea, bacteria and eukarya. Like pumping potassium in and outside of the cell, producing certain kind of metabolites etc. Isolation and characterisation of halotolerant bacteria and algae and their potential for biofuel production by jasem saleh almohsen msc. Biochemical and morphological characterization of the bacteria were carried out. In this study, a total of 28 different types of bacteria were isolated as pure culture on 3% nacl containing medium. Some nonbacterial organisms with thick cell walls e. The identification of salttolerant, or halophilic, pgprs has the potential to promote saline soilbased agriculture. Halophilic and halotolerant bacteria were isolated from soil samples of a japanese salt field, an environment where salt concentrations vary annually.
Sneathiella is named after the british microbiologist peter h. Halotolerant extremophile bacteria from the great salt. The bacteria isolates s 14 produced gummy except s1 colonies of different shape, size margin and elevation. A gramstainpositive, halotolerant, neutrophilic, rodshaped bacterium, strain mf38 t, was isolated from a salinealkaline soil in china and subjected to a polyphasic taxonomic characterization. Halotolerance is a relative term that refers to the ability to survive or thrive at salt concentrations higher than those necessary for growth. Get a printable copy pdf file of the complete article 857k, or click on a.
1451 228 798 329 1126 1076 1116 929 1534 511 230 162 266 1079 481 1486 1104 1309 732 1032 1308 876 1583 1508 1516 906 729 1339 1467 167 972 757 232 227 416 607 180 596